RECORD OF
STUDIES

Our studies are performed with utmost care

Below, please find a brief description to demonstrate results on some of our studies and clinical trials. If you’re interested in further details about the studies please contact us. The signing of a non-disclosure and confidentially agreement may be required.

Clinical trials and studies shown have all been carried out with the highest level of scientific rigour at some of the best equipped, independent facilities, universities and laboratories available.

PLASMA VOLUME CHANGE

Human Hydration – A double-blind, randomized cross-over clinical hydration study showed that drinking <33 treated water decreased the extent of dehydration in athletes and in 87% prevented dehydration. Close to 90% of subjects drinking <33 treated water showed an improvement over untreated water.

The results are significant showing <33 treated water provides superior hydration over untreated water. The results conclude (p<0.05) that <33 water will not only give better hydration; it also has a significant effect on preventing dehydration. The difference in blood plasma / blood volume is also directly related to a noticeable increase in performance as shown in various published clinical studies.

CALCIUM ION ACTIVITY

University Laboratory Study shows <33 treatment reduces calcium ion activity in water by almost 40%.

SOLUBILITY & SOLVATION / MICROCALORIMETRY

University Laboratory Study shows <33 treated water results in increased solubility, therefore improving the solvation.

Beneficially changes the thermodynamics of solvation less negative enthalpy for each injection water makes & breaks bonds when solvating dissolved ions & molecules.

The more heat evolved, the harder it is for water to retain important ions & electrolytes
<33 conditioned water
<33 reduces the heat evolved & helps keep electrolytes in solution

CELL HEALTH

University Study demonstrates the effect of <33 treatment on cells performed on frog oocytes (unfertilized egg cells).

Oocytes incepted in treated water for 5 consecutive days showed a significant difference in the membrane potentials of oocytes when compared with oocytes in the ‘control’ group.

WATER UPTAKE IN FLOWER STEMS

Uptake of water in cut flowers with treated water vs no-treated. <33 promotes water uptake in cut flowers providing better hydration.

COFFEE EXTRACTION

Effects of <33 treated water vs untreated water on coffee extraction.

MELATONIN

The effect of the <33 Technology and ‘sham’ disks on the production and secretion of melatonin from the Y79 cells were studied in five independent experiments, two of which were performed as double blind studies. The melatonin levels in each sample was measure after 12 hours of incubation of the Y79 cells with the two types of disks (<33 Technology and ‘sham’).

The results for each experiment as well as the summary of all studies where the melatonin levels for the <33 Technology samples are normalized to those for the ‘sham’ samples. Compared to the cells exposed to the ‘sham’ disks, those exposed to the <33 Technology disks resulted in a statistically significant (***P<0.0001, 2 way ANOVA) increase in melatonin levels. Experiments were repeated with different passage numbers.30 Incubation for more than 12 h did not result in increase in basal melatonin levels.

In this study, the effect of the <33 Technology on melatonin production and secretion in Y79 cells was investigated.

The results show that cells exposed to <33 Technology disks produce greater than 20% increase in melatonin levels compared to the ‘sham’ controls.

It has been suggested that <33 Technology improved sleep in a randomized double blind with placebo controlled study and our results may provide a rational explanation for this suggestion as the role of melatonin in promoting sleep is well accepted with this being the first study that scientifically shows exposure to <33 to cause a direct effect on melatonin secretion in Y79 human retinoblastoma cells.

WOUND HEALING

Effects on <33 technology on wound healing as shown in two clinical wound healing trials performed by Dr. Dirk Lange, Assistant Professor in the Department of Urologic Sciences at the University of British Columbia and Director of Basic Science Research at the Stone Centre at Vancouver General Hospital.

The clinical study results indicate that <33 accelerates Stage 3 of wound healing.

The results from this wound healing study demonstrate that exposure to <33 accelerates the third phase of wound healing and helps speed up the growth of new tissue.

READ MORE IN COSMETICS & CLINICAL

VITAMIN C LONGEVITY

Measuring the Degradation of Vitamin C Using UV-VIS Spectroscopy: An aqueous solution of vitamin C and oxalate was placed in multiple cuvettes. The absorption spectrum was measured for each cuvette for both placebo beads and <33 Technology beads. The graph below shows the spectra for both placebo and <33 beads. Four different wavelengths were used to monitor the degradation (260, 265, 270 and 275 nm).

ENDURANCE & RECOVERY

Fifteen subjects tested – repeated biceps isokinetic contractions
Conditions Placebo & <33

PLACEBO
<33
Exp.
0.021
0.019
Difference
11
T-Test
0.19
Standard Error (Torque)
0.023

Lower exp. coefficient means lower force decrease over time.

POSTURAL STABILITY

COP area – measure of postural stability
Lower COP area = higher stability

<33 decreased COP area by 32% compared to Placebo